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Biotechnology
Centre |
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| About
Centre
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| HISTORICAL
PERSPECTIVE OF THE CENTRE: |
Biotechnology Centre has
been established under the Department of Agril.
Botany in the year 2002 at main campus of Dr. Panjabrao
Deshmukh Krishi Vidyapeeth, Akola. Centre started
M. Sc. and Ph.D. degree programme in the subject
of Agricultural Biotechnology. With the intake capacity
of 12 students for M.Sc (Agril. Biotechnology) and
3 students for Ph.D. (Agril. Biotechnology).
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| Academic
Programmes
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At present this centre
follows the guidelines from ICAR and offers
different courses of M.Sc. and Ph.D. degree
programme in Agricultural Biotechnology, suiting
to the national need in public/ private sectors.
Syllabus proposed by
National Core Group, Education Division, ICAR,
New Delhi has been formally accepted by the
university.
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| Infrastructure
Facilities |
a) State of art laboratories:
| Plant
Genomics Laboratory |
Gradient
PCR (3 Units), Gel documentation system, SG
water purification system, Fume hood, Electrophoresis
equipments, Vacuum concentrator, Mix mate,
-80oC deep freezer, Bottle washing machine,
Ultra centrifuge . |
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| Plant Tissue
Culture & Transformation Laboratory |
Real time
PCR, Liquid handling system, Gene bombardment
gun, Environmental walk-in-chamber, Complete
set up for tissue culture. |
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| Value Addition
& Microbial Technology Laboratory |
Spray
dryer, High performance liquid chromatography(HPTLC),
Gas chromatography (GC), High performance
thin layer chromatography (HPTLC), Fourier
transform infrared spectroscopy (FTIR), High
end CCD attached research and zoom microscopes,
High end automatic fermenter. |
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| Plant Protection
Biotechnology Laboratory |
Gradient
PCR, Gel documentation system, Fume hood,
Electrophoresis equipments, Vacuum concentrator,
-80oC deep freezer, Protein purification machine
(FPLC), ELISA reader, Gel dryer, Iso Electric
Focusing(IEF) machine. |
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| Nanotechnology and Bioinformatics
Laboratory |
Basic bioinformatics
facility with collection of software |
b) Transgenic green
house and Net House facilities: 2
c) Lecture Hall- 2 No.
d) Seminar Hall-1 No
e) Internet/Computer facilities for the students
f) Central Library facility available for the staff
and students of this department
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Lecture Hall |
Seminar Hall |
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| Mandate
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| Biotechnology
Centre, Dr. PDKV., Akola is engaged in teaching, research
and extension activities in the field of Agricultural
Biotechnology. Centre is working with the following
objectives:
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| 1. |
To
foster multidisciplinary research collaboration and
networking in this important areas of agriculture
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| 2. |
To
strengthen and promote excellence in teaching in the
current aspects of biotechnology relevant to agriculture
with a focus on high quality postgraduate education |
| 3. |
Development
of human resources within the University by training
the staff members and students from other disciplines
of agriculture in the field of Biotechnology |
| 4. |
To
develop new and novel value added products, processes,
platform technologies and patents |
| 5. |
To
sustainably exploit India’s natural biodiversity
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| 6. |
To
facilitate the dissemination of information to end-users
in Agriculture |
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| Research
Activities |
| Research
Projects Completed: |
Source |
Name
of project |
Year |
Amount
(Rs.)
Lakhs
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Purpose |
| ICAR Catch-up grants |
Central Assistance |
2000-01 |
19.0
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Renovation of Biotechnology
laboratory |
| State Govt. VSDB, Board
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Establishment of Biotechnology
Lab. at Dr. PDKV., Akola |
2001-02 |
50.00
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Development and infrastructure
facilities |
| BRNS, DAE, GOI |
In vitro mutagenesis
for
desirable Agronomic and biochemical characters
in sugarcane
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2004-08 |
12.00 |
Isolated desirable mutants
for Vidarbha region of the Maharashtra |
| ICAR, N.Delhi |
Strengthening of Plant
Tissue Culture Laboratory |
2006-08 |
13.00 |
Strengthening of Plant
Tissue Culture Lab |
| State Government |
Education and Research
in biotechnology, tissue culture and genetic
engineering |
2006-09 |
500.00
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Strengthening of Biotechnology
Center |
| ICAR, N. Delhi |
Pigeonpea Genomics Initiative |
2006-10 |
32.28
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Development of mapping
population |
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Ongoing
Research Projects: |
Sr.
No. |
Name
of Research Project |
Scientists
involved |
| 1 |
Pigeonpea genomics initiative |
PI : Dr. R.S.Nandanwar
CoPI :
Dr.A.N.Patil
Dr.A.A.Akhare
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| 2 |
In-vitro mutagenesis
in sugarcane and its molecular characterization |
PI : Dr. E. R. Vaidya
CO-PI : Dr. P. G. Ingole |
| 3 |
Genomic approaches for
the selection of Mungbean Yellow Mosaic Virus
resistance trait in Glycine max (M) |
PI : Dr. M. P. Moharil
CO-PI :
Dr. R. S. Nandanwar
Dr. S. S. Mane
Dr. S. K. Dhapake
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| 4 |
Transgenic development
in safflower against safflower aphids using
lectin genes |
PI : Dr. M. S. Dudhare
Co-PI :
Dr.P.V.Jadhav
Dr.M.P.Moharil
Dr.P.N.Mane
Dr.S.N.Deshmukh
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| 5 |
Genetic improvement
of chickpea for pod borer using biotechnological
approaches |
PI : Mr. A. G. Deshmukh
CO-PI :
Dr.A.N.Patil
Mr.R.N.Wadaskar
Dr.M.S.Dudhare
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| 6 |
Study of biochemical
defense in soybean against Spodoptera litura |
PI : Mr.S.S.Munje
CO-PI : Dr.U.P.Barkhade
Dr.R.S.Nandanwar
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| 7 |
Agrobacterium mediated
transgenic development through direct organogenesis
in Pigeonpea |
PI : Dr. A. A.Akhare
CO-PI :
Dr.S.J.Gahukar
Dr.A.N.PAtil
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| 8 |
Improvement of soybean
for ‘No-podding syndrome’ using
genetic manipulation approach |
PI : Dr. P. V. Jadhav
CO-PI :
Dr.S.S.Mane
Dr.R.S.Nandanwar
Dr.M.S.Dudhare |
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INSTITUTIONAL COLLABORATION:
Experiments are undertaken in collaboration with different
crop research units and other University Departments
of Dr. PDKV., Akola and also at other national institute
like NRCPB, New Delhi; ICAR, New Delhi; ICRISAT,Hyderabad;
Bhabha Atomic Research Centre (BARC), Mumbai and NIPGR,
New Delhi. |
| Research
Achievements |
Developed
micropropogation protocols in banana, turmeric,
sarpagandha, gladiolus.
Standardized shoot tip grafting
(STG) technique in citrus
Developed Recombinant Inbred
Lines (RILs) and identified QTLs for SMD resistance
in pigeonpea under Pigeonpea Genomics Initiative
programme
In vitro mutagenised, selected
desirable sugarcane clones were developed and under
multilocation trials.
Micropropagation: Micropropagation
protocols have been developed for fruit crops viz.
Safed musli, Indian aloe, Sarpagandha and Turmeric
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Micropropagation of safed musli
Sarpagandha plants obtained
through micropropagation
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Embryo culture:
It was attempted for interspecific hybrid between
various cultivated and wild species of cotton (G.
arboreum, G. hirsutum, G. thurberi, etc.)
Various Stages of embryo culture
in cotton
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In vitro screening: Wheat
germplasm was screened for drought resistance through
callus culture.
In vitro mutagenesis: Sugarcane
cell lines treated with gamma rays were evaluated
for putative mutants for drought and salinity resistance
under in vitro simulated conditions.
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Various Stages of
In vitro Mutagenesis in Sugarcane
Cane size, shape and colour
variation observed in different mutants of sugarcane
Var. CO - 86032
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Characterization of the wheat
varieties/genotypes: Varieties/promising genotypes
developed by the university were characterized using
SSR markers.
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| Extension
Activities and Achievements
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Biotechnology Centre participates
in the Shivarpheri, technology demonstration day
for the farmers and AgroTech Exhibitions, Krishak
Vigyan Manch, field days and Krishi melawas arranged
by respective crop research unit.
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| Research
Recommendations (Approved in AGRESCO) |
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| Contact
Information |
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Officer Inhcharge
Biotechnology Centre
Department of Agril. Botany
Post Graduate Institute
Dr. Panjabrao Deshmukh Krishi Vidyapeeth,
Akola- 444 104
MS (India)
Phone and Fax No: 0724-2258239
Email: jambuvant11@yahoo.co.uk |
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